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Title: Q-PCR based bioburden assessment of drinking water throughout treatment and delivery to the International Space Station
Authors: Newcombe, David
Stuecker, Tara
La Duc, Myron
Venkateswaran, Kasthuri
Keywords: Q-PCR
International Space Station
Issue Date: 11-Jul-2005
Publisher: Pasadena, CA : Jet Propulsion Laboratory, National Aeronautics and Space Administration, 2005.'
Citation: 35th International Conference on Environmental Systems (ICES); 8th European Symposium on Space Environmental Control Systems (ESSECS), Rome, Italy, July 11, 2005.
Abstract: Previous studies indicated evidence of opportunistic pathogens samples obtained during missions to the International Space Station (ISS). This study utilized TaqMan quantitative PCR to determine specific gene abundance in potable and non-potable ISS waters. Probe and primer sets specific to the small subunit rRNA genes were used to elucidate overall bacterial rRNA gene numbers. while those specific for Burkholderia cepacia and Stenotrophomonas maltophilia were optimized and used to probe for the presence of these two opportunistic pathogens. This research builds upon previous microbial diversity studies of ISS water and demonstrates the utility of Q-PCR tool to examine water quality.
Appears in Collections:JPL TRS 1992+

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